LowAdhere Cytocaf®
Pack size: 25 mL, 1 mg/mL
Price: £100
Available from TCS Biosciences Ltd.
Features checklist
Recombinant protein polymer for non-adhesive cell culture
- Promotes smaller, rounder and more viable spheroids
- Supports a range of adherent mammalian cell types
- No background cell attachment to plastic or glass
- Compatible with multiple plate formats - no need for U-bottomed wells
- Reproducible results across experiments
Product description
LowAdhere Cytocaf® is a recombinant protein polymer that forms a biologically inert, non-adhesive coating on plastic and glass surfaces. When applied to culture wells, LowAdhere Cytocaf® prevents adherent mammalian cells from attaching to the surface, instead encouraging cell–cell interactions that promote the formation of uniform 3D structures, including spheroids and organoids.
LowAdhere Cytocaf® is compatible with a wide range of cell types and well formats, allowing selective coating of only the wells you need. This flexibility enables more efficient use of resources while maintaining high reproducibility. Cultures grown on LowAdhere Cytocaf®-coated wells exhibit improved structural properties—such as greater uniformity, circularity, and viability—without detectable background adhesion to the substrate.
Whether you're working in drug screening, tissue engineering, or advanced cell modelling, LowAdhere Cytocaf® offers a reliable and scalable solution for high-quality 3D cell culture.
References
- Roque, A. I., Soliakov, A., Birch, M. A., Philips, S. R., Shah, D. S. & Lakey, J. H. (2014). Reversible non-stick behaviour of a bacterial protein polymer provides a tuneable molecular mimic for cell and tissue engineering. Adv Mater 26, 2704-2709, 2616.
Data & specifications
| Test | Result |
|---|---|
| Bacterial growth | Negative |
| Fungal growth | Negative |
| Particulate examination | Negative |
| Mycoplasma | Negative |
Figure 1: HCT116 cells were seeded at a density of 1x10^4 cells/well into wells of an 96-well plate coated with LowAdhere Cytocaf®, then observed over 5 days using brightfield microscopy, where spheroid formation could be seen.
Figure 2: HEK293 cells were seeded at a density of 5x10^4 cells/well into wells of a 6-well plate that had either been left uncoated (A) or coated with LowAdhere Cytocaf® (B). A similar experiment was seeded on a commercially available low attachment plate (C). Cells were grown for 5 days then imaged using an EVOS light microscope. (D) A presto blue cell viability assay was performed on day 5 to measure the number of viable cells attached versus in suspension.