Fibronectin Cytocaf®
Pack size: 1 mL, 1 mg/mL
Price: £100
Available from TCS Biosciences Ltd.
Reliable Cell Attachment.
Defined Performance.
Reproducible Results.
For researchers working with adherent cell cultures, the coating surface is the foundation of every experiment. Cell attachment, morphology, phenotype and downstream assay performance are all influenced by the extracellular matrix environment. When coating materials are variable, undefined or difficult to standardise, experimental uncertainty can be introduced before the study even begins.
Fibronectin Cytocaf® is a recombinant, animal-free surface coating reagent designed to provide reliable cell attachment in a defined and consistent format. By combining the Cytocaf® protein-polymer scaffold with a genetically encoded fibronectin adhesion motif, it delivers biologically relevant cell-adhesion functionality while helping researchers reduce variability and improve reproducibility across cell culture workflows.
Why Choose Fibronectin Cytocaf®?
Reduce Surface Coating Variability
Move beyond animal-derived and poorly defined coating materials. Fibronectin Cytocaf® provides a controlled, reproducible coating environment that supports consistent culture conditions across experiments and between users.
Animal-Free and Defined
Manufactured using recombinant technology, Fibronectin Cytocaf® eliminates the variability and regulatory concerns associated with animal-derived extracellular matrix components.
Support Reliable Cell Culture
Designed to promote robust cell attachment across a range of adherent cell types, including mesenchymal stem cells (MSCs), helping researchers establish cultures with confidence.
Easy Integration into Existing Workflows
Supplied in water and compatible with standard plastic and glass cultureware, Fibronectin Cytocaf® can be incorporated into routine laboratory protocols using a simple 1-hour coating procedure.
Built for Reproducibility
The polymer-forming properties of the Cytocaf® scaffold help create a durable coating layer that supports routine experimentation and repeatable outcomes.
Key Benefits
✓ Defined recombinant coating reagent
✓ Animal-free composition
✓ Supports MSC and adherent cell culture workflows
✓ Promotes reliable cell attachment
✓ Simple 1-hour coating protocol
✓ Cost-effective alternative to traditional coating materials
✓ Minimal batch-to-batch variation
Applications
Fibronectin Cytocaf® is suitable for use with:
- Mesenchymal stem cells (MSCs)
- Dermal fibroblasts
- Macrophages
- Keratinocytes
- Cell lines inc.: U2OS, HCT116, HepG2, HEK293
Is your cell type not listed? Contact us!
Simple Workflow
Prepare
Dilute Fibronectin Cytocaf® to the recommended working concentration in sterile PBS and apply to non-tissue culture-treated cultureware.
Coat
Distribute the solution evenly across the culture surface to create a uniform coating layer.
Incubate
Incubate for at least 1 hour at room temperature.
Seed Cells
Remove excess coating solution and seed cells directly onto the prepared surface.
Culture
Grow cells using standard culture conditions and downstream workflows.
Frequently Asked Questions
Published Studies
- Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira-Duarte, A., Lakey, J. H. & Fulton, D. A. (2022). Exploiting Meltable Protein Hydrogels to Encapsulate and Culture Cells in 3D. Macromolecular Bioscience 22, 2200134
- Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira, A. M., Lakey, J. H. & Fulton, D. A. (2021). Hydrogels of engineered bacterial fimbriae can finely tune 2D human cell culture. Biomaterials Science 9, 2542-2552.
- Dura, G., Peters, D. T., Waller, H., Yemm, A. I., Perkins, N. D., Ferreira, A. M., Crespo-Cuadrado, M., Lakey, J. H. & Fulton, D. A. (2020). A Thermally Reformable Protein Polymer. Chem 6, 3132-3151.
- Roque, A. I., Soliakov, A., Birch, M. A., Philips, S. R., Shah, D. S. & Lakey, J. H. (2014). Reversible non-stick behaviour of a bacterial protein polymer provides a tuneable molecular mimic for cell and tissue engineering. Adv Mater 26, 2704-2709, 2616.