Vitronectin (Caf1-Vitronectin)

MarraBio Caf1 Vial

Features checklist

  • Provides adhesive surface: Ideal for cells such as iPSCs
  • Easy to handle: No need to keep cool, can be used at room temperature and above without denaturing or losing activity ​
  • Simple 1 hour protocol for coating plates
  • Free from animal material

Product description

Caf1 is a bacterial protein that associates into long, flexible and bioinert polymers. Bioactivity can be imparted by genetically encoding bioactive motifs into the protein’s sequence. Caf1 polymers are highly stable, modular and flexible.

Caf1-Vitronectin mimics the action of the extracellular matrix protein vitronectin and is adhesive for a range of cell types such as human mesenchymal stromal cells (MSCs) and induced pluripotent stem cells (iPSCs).

Cells grown on Caf1-Vitronectin display adherence and morphologies similar to those seen on recombinant vitronectin and Geltrex™

References

  1. Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira-Duarte, A., Lakey, J. H. & Fulton, D. A. (2022). Exploiting Meltable Protein Hydrogels to Encapsulate and Culture Cells in 3D. Macromolecular Bioscience 22, 2200134
  2. Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira, A. M., Lakey, J. H. & Fulton, D. A. (2021). Hydrogels of engineered bacterial fimbriae can finely tune 2D human cell culture. Biomaterials Science 9, 2542-2552.
  3. Dura, G., Peters, D. T., Waller, H., Yemm, A. I., Perkins, N. D., Ferreira, A. M., Crespo-Cuadrado, M., Lakey, J. H. & Fulton, D. A. (2020). A Thermally Reformable Protein Polymer. Chem 6, 3132-3151.
  4. Roque, A. I., Soliakov, A., Birch, M. A., Philips, S. R., Shah, D. S. & Lakey, J. H. (2014). Reversible non-stick behaviour of a bacterial protein polymer provides a tuneable molecular mimic for cell and tissue engineering. Adv Mater 26, 2704-2709, 2616.

Data & specifications

Test Result
Bacterial growth Negative
Fungal growth Negative
Particulate examination Negative
Endotoxin level < 40 EU/µg 
Caf1 Vitronectin Fig 1

Figure 1: SDS-PAGE showing purified Caf1-Vitronectin, heated (monomer) and unheated (polymer).

Caf1 Vitronectin Fig 2

Figure 2: (A) iPSCs derived from human dermal fibroblasts were cultured on non-TC plastic culture dishes coated with 2µg/cm² Caf1-Vtn. Images were taken at 10X magnification, 48 hours after seeding, by bright field microscopy. (B) Non-TC plastic was either left uncoated, coated with Caf1-WT or Caf1-Vtn batch 8 prior to seeding hDfa iPSCs in E8 Flex media supplemented with 10 µM ROCK inhibitor. After 24 hours media was exchanged to remove the inhibitor. A further 24 hours later, cell viability was measured using the Presto Blue cell viability assay, as per manufacturer’s specifications.