Fibronectin (Caf1-Fibronectin)

MarraBio Caf1 Vial

Features checklist

  • Provides adhesive surface: Ideal for cells such as MSCs
  • Easy to handle: No need to keep cool, can be used at room temperature and above without denaturing or losing activity ​
  • Simple 1 hour protocol for coating plates
  • Free from animal material

Product description

Caf1 is a bacterial protein that associates into long, flexible and bioinert polymers. Bioactivity can be imparted by genetically encoding bioactive motifs into the protein’s sequence. Caf1 polymers are highly stable, modular and flexible.

Caf1-Fibronectin mimics the action of the extracellular matrix protein fibronectin through a genetically encoded bioactive peptide that can bind to integrin receptors. This makes it adhesive for a range of cell types. These include, but are not limited to, human dermal fibroblasts, mesenchymal stromal cells (MSCs), epithelial-like cells (U2OS), hepatic cells (HEPG2), glioblastoma-derived cells (U118) and keratinocyte cells (CCD1106).

Cells grown on Caf1-Fibronectin display adherence and morphologies similar to those seen on recombinant Fibronectin, Geltrex™ and Matrigel™

References

  1. Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira-Duarte, A., Lakey, J. H. & Fulton, D. A. (2022). Exploiting Meltable Protein Hydrogels to Encapsulate and Culture Cells in 3D. Macromolecular Bioscience 22, 2200134
  2. Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira, A. M., Lakey, J. H. & Fulton, D. A. (2021). Hydrogels of engineered bacterial fimbriae can finely tune 2D human cell culture. Biomaterials Science 9, 2542-2552.
  3. Dura, G., Peters, D. T., Waller, H., Yemm, A. I., Perkins, N. D., Ferreira, A. M., Crespo-Cuadrado, M., Lakey, J. H. & Fulton, D. A. (2020). A Thermally Reformable Protein Polymer. Chem 6, 3132-3151.
  4. Roque, A. I., Soliakov, A., Birch, M. A., Philips, S. R., Shah, D. S. & Lakey, J. H. (2014). Reversible non-stick behaviour of a bacterial protein polymer provides a tuneable molecular mimic for cell and tissue engineering. Adv Mater 26, 2704-2709, 2616.

Data & specifications

Test Result
Bacterial growth Negative
Fungal growth Negative
Particulate examination Negative
Endotoxin level < 10 EU/µg 
Caf1 Fibronectin Fig 1

Figure 1: 15% SDS-PAGE showing purified Caf1-fibronectin, heated (monomer) and unheated (polymer).

Caf1 Fibronectin Fig 2

Figure 2: (A) Non-TC coated plastic was coated with 2µg/cm² Caf1-Fn batch 29. Human mesenchymal stromal cells were seeded in serum free media and grown for 72 hours. Images were taken by bright field microscopy at 10X magnification. (B) Human mesenchymal stromal cells were seeded in serum free media on uncoated, Caf1-WT coated or Caf1-Fn batch 29 coated non-TC plastic. The cells were grown for 24 hours prior to performing a Presto Blue cell viability assay, as per manufacturer’s specifications.