Our Products

Our proprietary engineered Cytocaf proteins provide an easy to use, ultra stable, scalable and xeno-free alternative to the extracellular matrix proteins and growth factors used in mammalian cell culture.

ECM Coatings

Fibronectin Cytocaf^®

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Vitronectin Cytocaf^®

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LamininB4 Cytocaf^®

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Validated Uses

Cell Type	Origin	Cells	Fibronectin Cytocaf®	Vitronectin Cytocaf®	LamininB4 Cytocaf®	Reference
Stem Cells	Human Bone Marrow MSCs	hBM-MSCs	✓		✓	[1]
	Induced Pluripotent Stem Cells	Human iPSCs		✓	✓	[1], [2]
	Induced Pluripotent Stem Cells	Porcine iPSCs		✓	✓	[3]
Muscle	Immortalized mouse skeletal muscle myoblast	C2C12			✓	[4]
Skin	Healthy Tissue	Neonatal Human Dermal Fibroblasts (hDFbs)	✓		✓	[4], [5]
Colon	Colorectal Cancer	HCT116	✓			[1]
Neural	Neuroblastoma	SH-SY5Y	✓			[4]
Neural	Healthy Tissue	iPSC derived neurones	✓			[4]
Liver	Hepatocarcinoma	HepG2	✓			[6]
Bone	Osteosarcoma	U2OS	✓			[6]
Immune	Mouse reticulum cell sarcoma	J774.A1 Macrophages	✓			[7]
Kidney	Adenovirus-transformed human embryonic kidney cells	HEK293	✓			[1]
Vascular	Umbilical Cord Blood	Endothelial Progenitor Cells		✓	✓	[4]
Vascular	Peripheral Blood	Endothelial Progenitor Cells		✓	✓	[4]

[1] Product Brochures (Request here)

[2] Engineering bacterial protein polymers to support human pluripotent stem cell growth and differentiation in culture. Creigh, A. R.; Waller, H.; Lakey, J. H.; Chrzanowska-Lightowlers, Z. M.; Lightowlers, R. N.; Peters, D. T..
bioRxiv 2024.04.29.591606; doi: https://doi.org/10.1101/2024.04.29.591606

[3] Application note: Cytocaf (Caf1) Polymers Enable Defined Adherent and Suspension Culture of Porcine iPSCs for Cultivated Meat Applications. Lacey, J.; Ross, J.; Nowell, A.; Reid, J.; Glen, A.; Waller, H.; Corbin, E.; Peters, D.T. (Request here)

[4] Unpublished data - contact us for more information.

[5] Hydrogels of engineered bacterial fimbriae can finely tune 2D human cell culture. Dura, G., Crespo-Cuadrado, M., Waller, H., Peters, D. T., Ferreira, A. M., Lakey, J. H., & Fulton, D. A., (2021). Biomaterials science, 9(7), 2542–2552. https://doi.org/10.1039/d0bm01966f

[6] A Thermally Reformable Protein Polymer. Dura, G; Peters, D. T.; Waller, H; Yemm, A. I.; Perkins, N. D.; Ferreira, A. M.; Crespo-Cuadrado, M.; Lakey, J. H.; Fulton, D. A. Chem, 2020; 6, 3132-3151

[7] Unraveling the molecular determinants of the anti-phagocytic protein cloak of plague bacteria. Peters D. T., Reifs A., Alonso-Caballero A., Madkour A., Waller H., Kenny B., Perez-Jimenez R., Lakey J.H. PLoS Pathog. 2022 Mar 31;18(3):e1010447. doi: 10.1371/journal.ppat.1010447. PMID: 35358289; PMCID: PMC9004762.